Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran

2 Department of Radiation Oncology, Isfahan University of Medical Science, Isfahan, Iran

Abstract

Objective: Gastric cancer (GC) is the fourth most common cancer worldwide, occurring in approximately two-thirds of developing countries, and is one of the leading causes of cancer deaths. The mitogen-activated protein kinase 14 (MAPK14) plays an important role in the conversion of extracellular stimuli to a wide range of cellular responses. The 5-hydroxytryptamine receptor 2B (HTR2B) increases the level of 5-HT; and as a result, platelet-derived 5-HT promotes tumor angiogenesis, tumor growth, and metastatic potential of cancer cells. Materials and Methods: Expression analysis was performed on 20 healthy cases and 20 cancerous cases for MAPK14 and 20 healthy cases and 19 cancerous cases for HTR2B using real-time quantitative PCR (qRT-PCR) assay. Polymorphisms were evaluated in 38 healthy cases and 30 cancerous cases for rs550352538, and 35 healthy cases and 35 cancerous cases for rs28763973 via tetra-primer amplification refractory mutation system PCR (T-ARMS PCR) technique. Results: No significant difference was observed in MAPK14 and HTR2B gene expression levels (P>0.05). In addition, there is no statistically significant association of different genotypes in rs28763973 from MAPK14 gene and rs550352538 from HTR2B gene with gastric cancer (P>0.05). Conclusions: No significant difference was noted between patient and healthy individuals in expression levels and gene polymorphisms; nevertheless, results may vary by significantly changing the gene pool or population size.

Keywords

Main Subjects

References

Browne, A., Göbel, A., Thiele, S., Hofbauer, L., Rauner, M., & Rachner, T. (2016). p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells. Cell death & disease, 7, e2119-e2119. doi:10.1038/cddis.2016.32.
Consortium, U. (2019). UniProt: a worldwide hub of protein knowledge. Nucleic acids research, 47, D506-D515. doi:10.1093/nar/gky1049.
Digklia, A., & Wagner, A. D. (2016). Advanced gastric cancer: Current treatment landscape and future perspectives. World journal of gastroenterology, 22, 2403-2414. doi:10.3748/wjg.v22.i8.2403.
Ebrahimi, M. E., Kordi-Tamandani, D. M., & Arish, M. (2016). A novel approach to investigation of the pathogenesis of pterygium based on assessment of promoter hyper-methylation and expression profile of CTLA4 gene: A credible report of CTLA4 gene expression in human eye tissue. Gene, 583, 130-133. doi:10.1016/j.gene.2016.02.027.
Eusebi, L. H., Telese, A., Marasco, G., Bazzoli, F., & Zagari, R. M. J. J. o. g. (2020). Gastric cancer prevention strategies: a global perspective. Journal of gastroenterology and hepatology, 35, 1495-1502. doi:10.1111/jgh.15037.
García-Hernández, L., García-Ortega, M. B., Ruiz-Alcalá, G., Carrillo, E., Marchal, J. A., & García, M. Á. (2022). The p38 MAPK Components and Modulators as Biomarkers and Molecular Targets in Cancer. International journal of molecular sciences, 23, 370. doi:10.3390/ijms23010370.
Johnston, F. M., & Beckman, M. (2019). Updates on management of gastric cancer. Current oncology reports, 21, 1-9. doi:10.1007/s11912-019-0820-4.
Karmakar, S., & Lal, G. (2021). Role of serotonin receptor signaling in cancer cells and anti-tumor immunity. Theranostics, 11, 5296–5312. doi:10.7150/thno.55986.
Katoh, H., & Ishikawa, S. (2021). Lifestyles, genetics, and future perspectives on gastric cancer in east Asian populations. Journal of human genetics, 66, 887-899. doi:10.1038/s10038-021-00960-8.
Li, Y., Kong, C., Wu, C., Wang, Y., Xu, B., Liang, S., & Ying, X. (2019). Knocking down of LINC01220 inhibits proliferation and induces apoptosis of endometrial carcinoma through silencing MAPK11. Bioscience reports, 39, BSR20181794. doi:10.1042/BSR20181794.
Padmanabhan, N., Ushijima, T., & Tan, P. (2017). How to stomach an epigenetic insult: the gastric cancer epigenome. Nature Reviews Gastroenterology & Hepatology, 14, 467-478. doi:10.1038/nrgastro.2017.53.
Peters, M. A., Meijer, C., Fehrmann, R. S., Walenkamp, A. M., Kema, I. P., de Vries, E. G., . . . Research, O. (2020). Serotonin and dopamine receptor expression in solid tumours including rare cancers. Pathology oncology research, 26, 1539-1547. doi:10.1007/s12253-019-00734-w.
Pruitt, K. D., Tatusova, T., & Maglott, D. R. J. N. a. r. (2005). NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic acids research, 33, D501-D504. doi:10.1093/nar/gki025.
Rawla, P., & Barsouk, A. (2019). Epidemiology of gastric cancer: global trends, risk factors and prevention. Przeglad gastroenterologiczny, 14, 26. doi:10.5114/pg.2018.80001.
Safran, M., Rosen, N., Twik, M., BarShir, R., Stein, T. I., Dahary, D., . . . Lancet, D. (2021). The GeneCards Suite. In I. Abugessaisa & T. Kasukawa (Eds.), Practical Guide to Life Science Databases (pp. 27-56). Singapore: Springer. doi:10.1007/978-981-16-5812-9_2.
Sexton, R. E., Al Hallak, M. N., Diab, M., & Azmi, A. S. (2020). Gastric cancer: a comprehensive review of current and future treatment strategies. Cancer metastasis reviews, 1-25. doi:10.1007/s10555-020-09925-3.
Sherry, S. T., Ward, M., & Sirotkin, K. (1999). dbSNP-database for single nucleotide polymorphisms and other classes of minor genetic variation. Genome research, 9, 677-679.
Sitarz, R., Skierucha, M., Mielko, J., Offerhaus, G. J. A., Maciejewski, R., & Polkowski, W. P. (2018). Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer management and research, 10, 239-248. doi:10.2147/CMAR.S149619.
Spandidos, A., Wang, X., Wang, H., & Seed, B. (2010). PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic acids research, 38, D792-D799. doi:10.1093/nar/gkp1005.
Sudo, T., Yagasaki, Y., Hama, H., Watanabe, N., & Osada, H. (2002). Exip, a new alternative splicing variant of p38 alpha, can induce an earlier onset of apoptosis in HeLa cells. Biochem Biophys Res Commun, 291, 838-843. doi:10.1006/bbrc.2002.6529.
Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin, 71, 209-249. doi:10.3322/caac.21660.
Tan, Z. (2019). Recent Advances in the Surgical Treatment of Advanced Gastric Cancer: A Review. Med Sci Monit, 25, 3537-3541. doi:10.12659/msm.916475.
Thrift, A. P., & El-Serag, H. B. (2020). Burden of gastric cancer. Clinical Gastroenterology and Hepatology, 18, 534-542. doi:10.1016/j.cgh.2019.07.045.
Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B. C., Remm, M., & Rozen, S. G. (2012). Primer3--new capabilities and interfaces. Nucleic Acids Res, 40, e115. doi:10.1093/nar/gks596.
Wang, F.-H., Zhang, X.-T., Li, Y.-F., Tang, L., Qu, X.-J., Ying, J.-E., . . . Xu, R.-H. (2021). The Chinese Society of Clinical Oncology (CSCO): Clinical guidelines for the diagnosis and treatment of gastric cancer, 2021. Cancer Commun (Lond), 41, 747-795. doi:10.1002/cac2.12193.
Yeoh, K. G., & Tan, P. (2021). Mapping the genomic diaspora of gastric cancer. Nature Reviews Cancer. doi:10.1038/s41568-021-00412-7.
Zhao, Y., Zhang, J., Cheng, A. S. L., Yu, J., To, K. F., & Kang, W. (2020). Gastric cancer: genome damaged by bugs. Oncogene, 39, 3427-3442. doi:10.1038/s41388-020-1241-4.
Journal of Epigenetics
Volume 3, Issue 2 - Serial Number 2
September 2022
Pages 1-8

Files

History

  • Receive Date: 31 January 2022
  • Revise Date: 16 July 2022
  • Accept Date: 28 July 2022

Share

How to cite

Statistics