Investigation of the expression of LncRNA/CCAT2 gene in patients with colorectal cancer

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran

2 Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract

Background and Aim: One of the most important topics in Colorectal Cancer (CRC) is identifying the genes that play a crucial role in cancer progression. Therefore, recognizing the important genes during the CRC progression is important as a biomarker for prognosis. Noncoding RNAs (ncRNAs), the major transcribed RNA molecules in animal cells, do not encode proteins and are mainly involved with the regulation of gene expression. The Colon Cancer Associated Transcript 2 (CCAT2) gene is transcribed to a LncRNA molecule and seems to be associated with several cancers. Methods: A total number of 20 colon cancer specimens and their corresponding healthy specimens from adjacent tissues were studied to investigate the expression of CCAT2. Total RNA was extracted, cDNA was synthesized, and finally, the expression of CCAT2 was determined in CRC and healthy tissues using Real-time Polymerase Chain Reaction (RT-PCR) assay. Results: The expression of CCAT2 in colorectal tumors and their adjacent healthy tissues was investigated. According to the results, the expression of CCAT2 in colorectal tumors and healthy tissues was significantly different. In other words, the expression of CCAT2 in cancer tissues was higher than the healthy tissues by 4.5 folds. Conclusion: The increased expression of LncRNA/CCAT2 is a common characteristic of colorectal cancer, and could be a good prognostic marker for this cancer.

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Akbari, F., Peymani, M., Salehzadeh, A. & Ghaedi, K. (2020). Integrative in silico and in vitro transcriptomics analysis revealed new lncRNAs related to intrinsic apoptotic genes in colorectal cancer. Cancer cell international, 20(1), 1-16.
Badmaev, V. (2009). Increased Growth Inhibitory Effects on Human Cancer Cells and Anti-inflammatory Potency of Shogaols from Zingibe... J Agric Food Chem, 57(22), 10645-10650.
Cabili, M.N., Trapnell, C., Goff, L., Koziol, M., Tazon-Vega, B., Regev, A. & Rinn, J.L. (2011). Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. Genes & development, 25(18), 1915-1927.
Chiu, H.S., Somvanshi, S., Patel, E., Chen, T.W., Singh, V.P., Zorman, B., Patil, S.L., Pan, Y., Chatterjee, S.S., Caesar-Johnson, S.J. & Demchok, J.A. (2018). Pan-cancer analysis of lncRNA regulation supports their targeting of cancer genes in each tumor context. Cell reports, 23(1), 297-312.
Danese, E. & Montagnana, M., 2017. Epigenetics of colorectal cancer: Emerging circulating diagnostic and prognostic biomarkers. Annals of translational medicine, 5(13).
Fernández, S.P., Díaz, S.P., Calviño, B.L., Santamaría, P.G., Pillado, T.S., Monreal, F.A., Maciá, F., Calavera, M.A.S., Macías, A.E., Ayerbes, M.V. & Pazos, A. (2010). Diagnosis delay and follow-up strategies in colorectal cancer. Prognosis implications: a study protocol. BMC cancer, 10(1), 1-6.
Foßelteder, J., Calin, G.A. & Pichler, M., 2018. Long non-coding RNA CCAT2 as a therapeutic target in colorectal cancer. Expert opinion on therapeutic targets, 22(12), 973-976.
Gupta, G.P. & Massagué, J., 2016. Cancer metastasis: building a framework. Cell, 127(4), 679-695.
Hauptman, N. & Glavac, D. (2013), Long Non-Coding RNA in Cancer. International Journal of Molecular Sciences, 14(1), 4655-4669.
Jasperson, K.W., Tuohy, T.M., Neklason, D.W. & Burt, R.W. (2010). Hereditary and familial colon cancer. Gastroenterology, 138(6), 2044-2058.
Lai, X.J. & Cheng, H.F. (2018). LncRNA colon cancer-associated transcript 1 (CCAT1) promotes proliferation and metastasis of ovarian cancer via miR-1290. Eur Rev Med Pharmacol Sci, 22(2), pp.322-328.
Ling, H., Spizzo, R., Atlasi, Y., Nicoloso, M., Shimizu, M., Redis, R. S., & Ivan, C. (2013). CCAT2, a novel noncoding RNA mapping to 8q24, underlies metastatic progression and chromosomal instability in colon cancer. Genome research23(9), 1446-1461.
Morlando, M. & Fatica, B. (2018), Alteration of Epigenetic Regulation by Long Noncoding RNAs in Cancer. international journal of Molecular Sciences, 570(19), 1-16.
Pourhoseingholi, M.A., Faghihzadeh, S., Hajizadeh, E., Abadi, A. & Zali, M.R. (2009). Bayesian estimation of colorectal cancer mortality in the presence of misclassification in Iran. Asian Pac J Cancer Prev, 10(4), 691-694.
Haggar, F.A. & Boushey, R.P. (2009). Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clinics in colon and rectal surgery, 22(4), 191-197.
Park, Y.J., Park, K.J., Park, J.G., Lee, K.U., Choe, K.J. & Kim, J.P. (1999). Prognostic factors in 2230 Korean colorectal cancer patients: analysis of consecutively operated cases. World journal of surgery, 23(7), 721-726.
Schmitz, S., Grote, P. & Herrmann, B. (2016), Mechanisms of long noncoding RNA function in development and disease. Cellular and Molecular Life Sciences, 73(1), 2491–2509.
Wang, L., Duan, W., Yan, S., Xie, Y. & Wang, C.  (2019). Circulating long non-coding RNA colon cancer-associated transcript 2 protected by exosome as a potential biomarker for colorectal cancer. Biomedicine & pharmacotherapy, 113, 108758.
World Health Organization International Agency for Research on Cancer (IARC))2020). GLOBOCAN 2020: estimated cancer incidence, mortality and prevalence worldwide in 2020
Xing, C., Sun, S.G., Yue, Z.Q. & Bai, F. (2021). Role of lncRNA LUCAT1 in cancer. Biomedicine & Pharmacotherapy, 134, 111158.
Yang, Y., Junjie, P., Sanjun, C. & Ma, Y., (2017). Long non-coding RNAs in colorectal cancer: progression and future directions. Journal of Cancer, 8(16), 3212.
Yu, Y., Nangia-Makker, P., Farhana, L., Rajendra, S. G., Levi, E. & Majumdar, A. P. (2015). miR-21 and miR-145 cooperation in regulation of colon cancer stem cells. Molecular cancer, 14(1), 1-11.
Yu, Y., Nangia-Makker, P., Farhana, L. & Majumdar, A. P. (2017). A novel mechanism of lncRNA and miRNA interaction: CCAT2 regulates miR-145 expression by suppressing its maturation process in colon cancer cells. Molecular cancer16(1), 1-11.
Volume 2, Issue 2 - Serial Number 2
October 2021
Pages 39-44
  • Receive Date: 26 December 2020
  • Revise Date: 06 January 2022
  • Accept Date: 08 February 2022
  • First Publish Date: 08 February 2022