Epi-drug-Loaded Niosomes in Combination with Blepharis Persica Improves Spinal Cord Injury in Male Rat

Document Type : Original Article

Authors

1 Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

2 Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

3 Graduated Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

1) Background: Taxol facilitates nerve regeneration but it has unwanted side-effects via the tumor suppressive function of miR-34a which induces XIST expression and affects paclitaxel administration. We aimed to examine the neuroprotective effect of Paclitaxel-loaded niosomes (PLNs) to enhance fertility by combining with the Belpharis Persica (B.P) extract in male Wistar rat model of spinal cord injury (SCI). 2) Methods: PLNs were prepared using the thin-film hydration method. After laminectomy at thoracic level (T9/T10) SCI was induced using the weight drop method. Rat treated with either PLNs (2.5mg/kg), PLNs (2.5mg/kg)+B.P extract (300mg/kg-14 days), B.P extract (300mg/kg-14 days), Normal, SCI (normal saline), and sham (laminectomy). 3) Results: The transmission electron microscopy TEM and laser diffraction methods were confirmed the existence of spherical vesicles with feature sizes about 200 nm. The various behavioral, pathological and molecular parameters (CATSPER gene expression) were distinguished. SCI resulted in a significant decrease (P<0.05) in sperm motility, behavioral skills, and CATSPER genes expression were all regenerated by treatment with combination therapy (PLNs+B.P extract) in comparison to the others. Besides, the increased in sperm motility, and CATSPER genes expression were significantly (P<0.05) demonstrated in PLNs and B.P treated groups. 4) Conclusion: Taken together, the results indicated that the PLNs and B.P groups showed a significant increase in sperm parameters, but combination therapy caused the greater recovery of both gene expression and motor functions to decrease the neurological deficits no serious adverse reactions and enhanced fertility.

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References

Afsar, T., Razak, S., & Almajwal, A. (2017). Acacia hydaspica ethyl acetate extract protects against cisplatin-induced DNA damage, oxidative stress and testicular injuries in adult male rats. BMC cancer, 17(1), 1-14.
"Aghaabbasi, K., Askari, N., Kumleh, H. H., Torkzadeh-Mahani, M., & Ramzani-Ghara, A. (2020). The Blepharis persica seed hydroalcoholic extract synergistically enhances the apoptotic effect of doxorubicin in human colon cancer and gastric cancer cells. Molecular Biology Reports, 47(2), 843-853."
Anderson, R., Moses, R., Lenherr, S., Hotaling, J. M., & Myers, J. (2018). Spinal cord injury and male infertility—a review of current literature, knowledge gaps, and future research. Translational andrology and urology, 7(Suppl 3), S373.
Antimisiaris, S. G., Marazioti, A., Kannavou, M., Natsaridis, E., Gkartziou, F., Kogkos, G., & Mourtas, S. (2021). Overcoming barriers by local drug delivery with liposomes. Advanced Drug Delivery Reviews.
"Baas, P. W. (2014). Beyond taxol: microtubule-based strategies for promoting nerve regeneration after injury. Neural regeneration research, 9(13), 1265."
"Cao, H. H., Zhang, Z. F., Wang, X. F., & Liu, T. X. (2018). Nutrition versus defense: Why Myzus persicae (green peach aphid) prefers and performs better on young leaves of cabbage. PloS one, 13(4), e0196219."
Davaa, G., Hong, J. Y., Kim, T. U., Lee, S. J., Kim, S. Y., Hong, K., & Hyun, J. K. (2021). Exercise Ameliorates Spinal Cord Injury by Changing DNA Methylation. Cells, 10(1), 143.
El Shihy, S. A., Abdeltawab, R. E., & El Tayebi, H. M. (2020). 131P Abrogation of paclitaxel chemo-resistance via epigenetic immune checkpoints regulation in triple negative breast cancer. Annals of Oncology, 31, S59.
"Georgens, C., Weyermann, J., & Zimmer, A. (2005). Recombinant virus like particles as drug delivery system. Current pharmaceutical biotechnology, 6(1), 49-55."
"Heidarizadi, S., Abbasi, N., Asadollahi, K., Rezaei, S., Moayeri, A., & Azizi, M. (2017). Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats. Journal of Basic Research in Medical Sciences, 4(1), 30-36."
Hong, J. Y., Davaa, G., Yoo, H., Hong, K., & Hyun, J. K. (2020). Ascorbic acid promotes functional restoration after spinal cord injury partly by epigenetic modulation. Cells, 9(5), 1310.
"Huang, H. F. S., Linsenmeyer, T. A., Anesetti, R., Giglio, W., Ottenweller, J. E., & Pogach, L. (1998). Suppression and recovery of spermatogenesis following spinal cord injury in the rat. Journal of andrology, 19(1), 72-80."
"Kamei, J., Hayashi, S., Sakai, A., Nakanishi, Y., Kai, M., Ikegami, M., & Ikeda, H. (2017). Rikkunshito prevents paclitaxel-induced peripheral neuropathy through the suppression of the nuclear factor kappa B (NF?B) phosphorylation in spinal cord of mice. PloS one, 12(2), e0171819."
Li, R., Li, D., Wu, C., Ye, L., Wu, Y., Yuan, Y., ... & Xiao, J. (2020). Nerve growth factor activates autophagy in Schwann cells to enhance myelin debris clearance and to expedite nerve regeneration. Theranostics, 10(4), 1649.
Li, X., Fan, C., Xiao, Z., Zhao, Y., Zhang, H., Sun, J., ... & Dai, J. (2018). A collagen microchannel scaffold carrying paclitaxel-liposomes induces neuronal differentiation of neural stem cells through Wnt/β-catenin signaling for spinal cord injury repair. Biomaterials, 183, 114-127.
Malcicka, M., Visser, B., & Ellers, J. (2018). An evolutionary perspective on linoleic acid synthesis in animals. Evolutionary biology, 45(1), 15-26.
Matczak, M., Marchelak, A., Michel, P., Owczarek, A., Piszczan, A., Kolodziejczyk-Czepas, J., ... & Olszewska, M. A. (2018). Sorbus domestica L. leaf extracts as functional products: phytochemical profiling, cellular safety, pro-inflammatory enzymes inhibition and protective effects against oxidative stress in vitro. Journal of Functional Foods, 40, 207-218.
Nowicka-Bauer, K., & Szymczak-Cendlak, M. (2021). Structure and Function of Ion Channels Regulating Sperm Motility—An Overview. International Journal of Molecular Sciences, 22(6), 3259.
Oyelowo, O. T., & Bolarinwa, A. F. (2020). Early-life exposure to omega-9 monounsaturated fatty acid results in gonadal-regression and elevated stress levels in pubertal male rat. Songklanakarin Journal of Science & Technology, 42(6).
Penas, C., & Navarro, X. (2018). Epigenetic modifications associated to neuroinflammation and neuropathic pain after neural trauma. Frontiers in cellular neuroscience, 12, 158.
"Radojicic, M., Nistor, G., & Keirstead, H. S. (2007). Ascending central canal dilation and progressive ependymal disruption in a contusion model of rodent chronic spinal cord injury. BMC neurology, 7(1), 30."
"Sabry, S., Okda, T., & Hasan, A. (2021). Formulation, characterization, and evaluation of the anti-tumor activity of nanosized galangin loaded niosomes on chemically induced hepatocellular carcinoma in rats. Journal of Drug Delivery Science and Technology, 61, 102163. "
"Scripture, C. D., Figg, W. D., & Sparreboom, A. (2006). Peripheral neuropathy induced by paclitaxel: recent insights and future perspectives. Current neuropharmacology, 4(2), 165-172."
"Sinha, V., Elliott, S., Ibrahim, E., Lynne, C. M., & Brackett, N. L. (2017). Reproductive health of men with spinal cord injury. Topics in Spinal Cord Injury Rehabilitation, 23(1), 31-41."
Sun, X. H., Zhu, Y. Y., Wang, L., Liu, H. L., Ling, Y., Li, Z. L., & Sun, L. B. (2017). The Catsper channel and its roles in male fertility: a systematic review. Reproductive Biology and Endocrinology, 15(1), 1-12.
Ubah, S. A., Agbonu, O. A., Columbus, P. K., Abah, K. O., Chibuogwu, I. C., Abalaka, S. E., ... & Ajayi, I. E. (2021). Effects of date fruit (Phoenix dactylifera) on sperm cell morphology and reproductive hormonal profiles in cypermethrin-induced male infertility in Wister rats. Scientific African, 11, e00713.
Washburn, R. L., Hibler, T., Thompson, L. A., Kaur, G., & Dufour, J. M. (2021, April). Therapeutic application of Sertoli cells for treatment of various diseases. In Seminars in Cell & Developmental Biology. Academic Press.
"Westermann, S., & Weber, K. (2003). Post-translational modifications regulate microtubule function. Nature reviews Molecular cell biology, 4(12), 938-948."
"Witte, H., Neukirchen, D., & Bradke, F. (2008). Microtubule stabilization specifies initial neuronal polarization. Journal of cell biology, 180(3), 619-632."
"Yin, W., Li, X., Zhao, Y., Tan, J., Wu, S., Cao, Y. Meng, L. (2018). Taxol-modified collagen scaffold implantation promotes functional recovery after long-distance spinal cord complete transection in canines. Biomaterials science, 6(5), 1099-1108."

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History

  • Receive Date: 25 July 2021
  • Revise Date: 22 December 2021
  • Accept Date: 23 January 2022

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