Heterosis and Combining Ability Evaluation in Different Tomato Inbred-lines for Salinity Tolerance During the Seedling Stage

Document Type : Original Article

Authors

1 Department of Plant Production, University of Torbat Heydarieh

2 Department of horticulture, Ferdowsi University of Mashhad

3 Department of Biotechnology, Ferdowsi University of Mashhad

Abstract

Vast numbers of studies on tomato breeding have been performed for resistance to environmental stresses and pests. In genetic investigation and breeding of tomato, it is tried to use tomato lines with the most variance on morphological and agronomical traits to obtain better results, like maximum heterosis on F1 hybrids. In this survey combining ability and heterosis of 7 inbred lines (ME, KaLN3, Fla, CT6, LA3770, R2-05, and DB) of tomato and their F1 hybrids were investigated for salinity tolerance during the seedling stage using the diallel-cross. Therefore, seed germination percentage, seedling emergence percent and rate under salt-stress, tested in a complete randomized design with four replications. Results show that the reciprocal effects and SCA  were significant for all traits on the probability of 1 %. Analysis of Wr-Vr regression also shown that seed germination percentage and seedling emergence percentage and rate under salt stress controlled by over-dominance effects. The best line for resistance to salt stress in juvenility was R205, which has the most GCA  for all three traits connected to salt resistance. It may be beneficial to combine this line as a parental line in breeding programs for increasing salinity tolerance of tomato during the seedling stage.

Keywords


Al-Busaidi, A., Al-Rawahy, S.,& Ahmed, M., (2009). Response of Different Tomato Cultivars to Diluted Seawater Salinity. Asian Journal of Crop Science 1, 77-86.
Al-Harbi, A. R., Wahb-Allah, M. A., & Abu-Muriefah, S. S. (2008). 'Salinity and Nitrogen Level Affects Germination, Emergence, and Seedling Growth of Tomato. International Journal of Vegetable Science, 14, 380-392.
Arrillaga, I., Gil Mascarell, R., Gisbert, C., Sales, E., Montesinos, C., Serrano, R., & Moreno, V. (1998). Expression of the yeast HAL2 gene in tomato increases the in vitro salt tolerance of transgenic progenies. Plant science , 136, 219-226.
Chen, Z., Shabala, S., Mendham, N., Newman, I., Zhang, G.,            & Zhou, M. (2008), Combining Ability of Salinity Tolerance on the Basis of NaCl‐Induced K+ Flux from Roots of Barley. DOI:10.2135/cropsci2007.10.0557. Crop Science, 48: 1382-1388.
Farzaneh, A., (2012). Estimation of the ability to combine and heterozygous for valuable agricultural traits in nine tomato lines using the dial method. Master Thesis, Ferdowsi University of Mashhad.
Foolad, M. R. (1997). Genetic basis of physiological traits related to salt tolerance in tomato, Lycopersicon esculentum Mill. Plant Breeding, 116, 53-58.
Foolad, M. R. (1999). Comparison of salt tolerance during seed germination and vegetative growth in tomato by QTL mapping. Genome, 42, 727-734.
Foolad, M. R. (2004). Recent Advances in Genetics of Salt Tolerance in Tomato. Plant Cell, Tissue, and Organ Culture, 76, 101-119.
Gisbert, C., Rus, A. M., Bolarín, M. C., Coronado, J. M., Arrillaga, I., Montesinos, C., Caro, M., Serrano, R., & Moreno, V.  (2000). The yeast HAL1 gene improves salt tolerance of transgenic tomato. Plant physiology, 123.
Kaveh, H., Nemati, H.,  Farsi, M., & Vatandoost Jartoodeh, S. (2011). How Salinity Affect Germination and Emergence of Tomato Lines. J. BIOL. ENVIRON. SCI. 5, 159-163.
Li, J., Liu, L., Bai, Y., Zhang, P., Finkers, R., Du, Y., Visser, R.,   & van Heusden, A., 2011. Seedling salt tolerance in tomato. Euphytica 178, 403-414.
Moghaieb, R. E. A., Tanaka, N., Saneoka, H., Hussein, H. A., Yousef, S. S., Ewada, M. A. F., Aly, M. A. M., & Fujita, K. (2000). Expression of betaine aldehyde dehydrogenase gene in transgenic tomato hairy roots leads to the accumulation of glycine betaine and contributes to the maintenance of the osmotic potential under salt stress. Soil science plant nutrition, 46, 873-883.
Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell & Environment, 25, 239-250.
Nemati, H., Nazdar, T.,  Azizi, M., &  Arouiee, H.  (2010). The Effect of Seed Extraction Methods on Seed Quality of Two Cultivar's Tomato (Solanum lycopersicum L.). Pakistan Journal of Biological Sciences, 13, 814-820.
Nemoto, K., Ukai, Y., Tang, D. Q., Kasai, Y., & Morita, M. (2004). Inheritance of early elongation ability in floating rice revealed by diallel and QTL analyses. TAG Theoretical and Applied Genetics, 109, 42-47.
Nuez, F., & Prohens., J. (2008). Handbook of plant breeding. vegetables II. (Ed), 2008. Handbook of plant breeding, Vegetables II. Springer, New york.
Omholt, S. W., Plahte, E.,  Oyehaug, L., & KeFang, X. (2000). Gene regulatory networks generating the phenomena of additivity, dominance, and epistasis. Genetics, 155, 969-980.
Peralta, I. E., Knapp, S., & Spooner, D. M. (2005). New Species of Wild Tomatoes (Solanum Section Lycopersicon: Solanaceae) from Northern Peru. Systematic Botany, 30, 424-434.
Sekhar, L., Prakash, B. G., Salimath, P. M., Channayya, M., Hiremath, P., Sridevi, O., & Patil, A. A. (2010). Implication of heterosis and combining ability among productive Single cross Hybrid in tomato. Electronic Jornal of Plant Breeding, 1(4): 706-711.
Titok, V. V., Lemesh, V. A., Rusinova, O.V., &  Podlisskikh, V. L. (1994). Leaf area, chlorophyll content and biomass of tomato plants and their heterotic hybrids under in vitro culture. Photosynthetica, 30, 255-260.
Zengin, S., Kabaş, A., Oğuz, A., Eren, A., & Polat, E. (2016). Determining of general combining ability for yield, quality, and some other traits of tomato (Solanum lycopersicum L.) inbred lines. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi , 28(1), 0-0. Retrieved from https://dergipark.org.tr/en/pub/akdenizfderg/ issue/ 25317/267420
Zhang, H. X., & Blumwald, E. (2001). Transgenic salt-tolerance tomato plants accumulate salt in foliage but not in fruit. Nature Biotechnology, 19.
Volume 2, Issue 1
January 2021
Pages 7-14
  • Receive Date: 01 October 2019
  • Revise Date: 01 October 2020
  • Accept Date: 11 October 2020
  • First Publish Date: 01 January 2021