Analysis of Cell-Free DNA (as a genetic index for embryo quality)in an Iranian Women Undergoing IVF

Document Type : Original Article


1 Department of Biology, central Tehran branch, Islamic Azad University, Tehran, Iran

2 Infertility center of Tehran Yas hospital, medical school of Tehran University of medical science, Tehran, Iran

3 Kowsar Human Genetics Research Institute, Yas medical Genetics Lab, Tehran, Iran


1) Background: In vitro fertilization (IVF) is offered as a treatment for infertility in women.
. The Cell free DNA in plasma/follicles were suggested as a biomarker for embryo quality in
IVF. Higher the levels of Cell-free DNA indicate poorer embryo quality in the follicular fluid
during IVF. Therefore, this study was designed to examine the cell-free DNA (cfDNA) in
plasma and Fluid-follicle of women undergoing IVF-embryo transfer. 2) Methods: The study
examined 100 women undergoing IVF treatment in an IVF unit in Yas Hospital of Tehran
University of Medical Sciences in Tehran-Iran. Cell-free DNA was extracted from plasma
and follicular fluid of patients using the NucleoSpin® Extraction Kit. The total cfDNA was
examined by qPCR for ALBUMIN and GAPDH genes. 3) Results: we did not find a
statistically significant association among the variables such as CT-plasma, CT-follicular
fluid, delta-CT and average of these data between two groups (including patients who had a
successful IVF cycle and those who had a failed IVF cycle). 4) Conclusion: Plasma and
follicular fluid cfDNA may reflect the presence of factors which interfere with embryo
implantation. Further research is required to determine the usefulness of cfDNA as a
biomarker of IVF outcome and to examine the underlying pathologies as potential sources
for increased plasma/follicular fluid cfDNA concentrations.


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